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fluorescence lifetime imaging microscopy


Identification code: 300390593



Alternative term/s

  • FLIM
  • fluorescence lifetime imaging microscopy
  • microscopy, fluorescence lifetime imaging

Anotations

Scope note:

Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique for producing molecular images based on the fluorescence lifetime (the average time that the molecule spends in the excited state) from a fluorescent sample.The lifetime of the fluorophore signal, rather than its intensity, is used to create the image in FLIM. The fluorescence lifetime can function as a molecular stopwatch to observe a variety of events.The technique is thus particularly suitable for live-cell imaging.

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